说明书
MSDS产品描述：Recombinant rabbit monoclonal antibody HA-tag免疫原：KLH-conjugated synthetic peptide YPYDVPDYA.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Monoclonal产品形式：Liquid in PBS, pH 7.3, 50% glycerol, and 0.05% Proclin300.稀释比：WB (1/5000 - 1/20000), IF/IC (1/300 - 1/1000), IP (1/50 - 1/200)相关名称：HA epitope tag; hemagglutinin
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Anti-HA-tag Antibody against HEK293T cells transfected with vector overexpressing HA tag (A). -
Immunofluorescent analysis of HA-Tag staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). -
Immunoprecipitation of HA-tagged protein from untransfected (A) and HEK293T cells transfected with vector overexpressing HA tag (B), using Recombinant Anti-HA-tag Rabbit mAb.