产品描述：Monoclonal Secondary Antibody to Rabbit IgG HRP labeled免疫原：Rabbit IgG纯化方式：The antibody is isolated from cell culture by protein A/G immunoaffinity chromatography.特异性：The antibody is a conformation-specific secondary antibody that only reacts with native rabbit IgG and does not bind to the denatured and reduced rabbit IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured IgG light and heavy chains of the primary antibody used for IP will not be recognised.克隆类型：Monoclonal标记物：HRP产品形式：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.05% Proclin稀释比：WB (1/500 - 1/2000) 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Immunoprecipitating GAPDH in Hela whole cell lysate with rabbit anti-GAPDH antibody. Western blot was performed on the immunoprecipitate using anti-GAPDH antibody, and followed by the normal Anti-Rabbit IgG - HRP and Anti-Rabbit IgG - HRP For IP/CoIP. Lane A and D (Input): Hela whole cell lysate; Lane B and E (-): Rabbit IgG Isotype Control instead of GAPDH antibody in Hela whole cell lysate; Lane C and F (+): GAPDH antibody IP in Hela whole cell lysate.