产品描述：Goat Polyclonal Secondary Antibody to Rabbit IgG (H&L) - AcalephFluor488免疫原：Rabbit IgG纯化方式：The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.特异性：By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG. No antibody was detected against non immunoglobulin serum proteins.克隆类型：Polyclonal标记物：AcalephFluor488产品形式：1 mg/ml. Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide稀释比：IH (1/100 - 1/1000), IF (1/100 - 1/1000), FC (1/1000 - 1/4000), E (Use at an assay dependent concentration) 检测平台：Ex/Em = 490/515 nm储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Line colors represent the approximate visible colors of the wavelength maxima.
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  Immunofluorescent analysis of beta Tubulin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST. The secondary antibody (green) was Goat Anti-Rabbit IgG (H&L) - AcalephFluor488 in PBS at room temperature in the dark.