说明书
MSDS产品描述：Rabbit polyclonal antibody to ALIX免疫原：Recombinant full length protein of human ALIX纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：PDCD6IP相关名称：AIP1; ALIX; KIAA1375; Programmed cell death 6-interacting protein; PDCD6-interacting protein; ALG-2-interacting protein 1; ALG-2-interacting protein X; Hp95
基因编号(人)：
10015;
基因编号(小鼠)：
18571;
基因编号(大鼠)：
501083;
蛋白编号(人)：
Q8WUM4;
蛋白编号(小鼠)：
Q9WU78;
蛋白编号(大鼠)：
Q9QZA2;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ALIX expression in Jurkat (A) whole cell lysates. (Predicted band size: 30; 96 kD; Observed band size: 105 kD) -
Immunofluorescent analysis of ALIX staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Palmitic acid differently modulates extracellular vesicles and cellular fatty acid composition of SGBS adipocytes without impairing their insulin signaling