产品描述：Rabbit polyclonal antibody to NBC1免疫原：Recombinant full length protein of human NBC1纯化方式：The antibody was purified by immunogen affinity chromatography.特异性：Recognizes endogenous levels of NBC1 protein.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：SLC4A4相关名称：NBC; NBC1; NBCE1; Electrogenic sodium bicarbonate cotransporter 1; Sodium bicarbonate cotransporter; Na(+)/HCO3(-) cotransporter; Solute carrier family 4 member 4; kNBC1 基因编号(人)： 8671; 基因编号(小鼠)： 54403; 基因编号(大鼠)： 84484; 蛋白编号(人)： Q9Y6R1; 蛋白编号(小鼠)： O88343; 蛋白编号(大鼠)： Q9JI66; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NBC1 expression in PC3 (A), A549 (B) whole cell lysates. (Predicted band size: 72; 112; 116; 121; 123 kD; Observed band size: 135 kD)
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  Immunofluorescent analysis of NBC1 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).