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CSA9104
  • Immunohistochemical analysis staining in human liver carcinoma formalin fixed paraffin-embedded tissue section. The section was pre-treated using pressure cooker heat antigen retrieval with sodium citrate buffer (0.01M, pH=6) for 3 minutes. The section was detected using mouse primary antibody, and Goat Anti-Mouse IgG (H&L)-HRP polymer (Ready to use). The section was then counterstained with haematoxylin and mounted with Neutral Gum.
产品名称:Goat Anti-Mouse IgG (H&L)-HRP Polymer (Ready to use)
货号:CSA9104
来源:Goat
反应物种:M
实验应用:E, WB, IH, IC
*反应物种注解:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*实验应用注解:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
规格
价格(元)
10 ml
900
100 ml
7000
Ship in 3 days
产品描述:Ready to use Goat Polyclonal Secondary Antibody to Mouse IgG (H&L) HRP polymer labled
免疫原:Mouse IgG
纯化方式:Goat Polyclonal Secondary Antibody to Mouse IgG (H&L) have been cross-adsorbed against IgG from bovine, goat, horse, rabbit and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.
克隆类型:Polyclonal
标记物:HRP Polymer
产品形式:Liquid in PBS, pH 7.2, containing 1% BSA, 0.05% Proclin
稀释比:Ready to use
使用指导:Goat Anti-Mouse IgG (H&L)-HRP polymer (Ready to use) is a ONE-step polymer system that provides increased sensitivity, time savings and detection simplicity. This product allows the use of less antibody to obtain better signal-to-noise ratios. This system is also biotin-free, which eliminates background staining found with traditional biotin-based detection methods.
储存效期:Shipped and store at 4°C for one year.
  • Immunohistochemical analysis staining in human liver carcinoma formalin fixed paraffin-embedded tissue section. The section was pre-treated using pressure cooker heat antigen retrieval with sodium citrate buffer (0.01M, pH=6) for 3 minutes. The section was detected using mouse primary antibody, and Goat Anti-Mouse IgG (H&L)-HRP polymer (Ready to use). The section was then counterstained with haematoxylin and mounted with Neutral Gum.
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