产品描述：Goat Polyclonal Secondary Antibody to Rabbit IgG (H&L) - AcalephFluor488免疫原：Rabbit IgG纯化方式：The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.克隆类型：Polyclonal标记物：AcalephFluor488产品形式：1 mg/ml. Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide稀释比：IH (1/100 - 1/1000), IF (1/100 - 1/1000), FC (1/1000 - 1/4000), E (Use at an assay dependent concentration) 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.检测平台：Ex/Em = 490/515 nm

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  Line colors represent the approximate visible colors of the wavelength maxima.
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  Immunofluorescent analysis of beta Tubulin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST. The secondary antibody (green) was Goat Anti-Rabbit IgG (H&L) - AcalephFluor488 in PBS at room temperature in the dark.