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CRJ7577
产品名称:SLCO4C1 siRNA (Human)
货号:CRJ7577
来源:Synthetic
反应物种:H
实验应用:RNAi
*反应物种注解:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*实验应用注解:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
规格
价格(元)
15 nmol
3400
30 nmol
5100
Ship in 2 weeks
产品描述:siRNA to inhibit SLCO4C1 expression using RNA interference
产品形式:Lyophilized powder
基因名称:SLCO4C1
相关名称:OATP4C1; OATPX; SLC21A20; Solute carrier organic anion transporter family member 4C1; OATP-H; Organic anion transporter M1; OATP-M1; Solute carrier family 21 member 20
基因编号(人): 353189;
蛋白编号(人): Q6ZQN7;
纯度:> 97%
使用指导:We recommends transfection with 10 nM - 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For example, resuspend one tube of 5 nmol siRNA oligo in 250 μl of DEPC water to get a final concentration of 20 μM.
质量控制:Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
组分:We offers pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLCO4C1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot.
储存效期:Shipped at 4 °C. Store at -20 °C for one year.
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