产品描述:Rabbit polyclonal antibody to COPG2免疫原:Recombinant fusion protein of human COPG2. The exact sequence is proprietary.纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)基因名称:COPG2相关名称:Coatomer subunit gamma-2; Gamma-2-coat protein; Gamma-2-COP
基因编号(人):
26958;
基因编号(小鼠):
54160;
蛋白编号(人):
Q9UBF2;
蛋白编号(小鼠):
Q9QXK3;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of COPG2 expression in mouse heart (A), mouse kidney (B) whole cell lysates. (Predicted band size: 81; 97 kD; Observed band size: 105 kD) -
Immunofluorescent analysis of COPG2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.


说明书
MSDS