产品描述：Rabbit polyclonal antibody to CLCA1免疫原：Recombinant fusion protein of human CLCA1. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)基因名称：CLCA1相关名称：CACC1; Calcium-activated chloride channel regulator 1; Calcium-activated chloride channel family member 1; hCLCA1; Calcium-activated chloride channel protein 1; CaCC-1; hCaCC-1 基因编号(人)： 1179; 基因编号(小鼠)： 23844; 蛋白编号(人)： A8K7I4; 蛋白编号(小鼠)： Q9D7Z6; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CLCA1 expression in mouse uterus (A), rat small intestine (B) whole cell lysates. (Predicted band size: 100 kD; Observed band size: 85 kD)
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  Immunofluorescent analysis of CLCA1 staining in human colon cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.