产品描述：Rabbit polyclonal antibody to CKMT1B免疫原：Recombinant fusion protein of human CKMT1B. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IP (1/10 - 1/50)基因名称：CKMT1B相关名称：CKMT; CKMT; Creatine kinase U-type mitochondrial; Acidic-type mitochondrial creatine kinase; Mia-CK; Ubiquitous mitochondrial creatine kinase; U-MtCK 基因编号(人)： 1159; 548596; 基因编号(小鼠)： 12716; 蛋白编号(人)： P12532; 蛋白编号(小鼠)： P30275; 蛋白编号(大鼠)： P25809; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CKMT1B expression in mouse kidney (A), rat brain (B) whole cell lysates. (Predicted band size: 47; 50 kD; Observed band size: 42 kD)
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  Immunofluorescent analysis of CKMT1B staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.