说明书
MSDS产品描述：Rabbit polyclonal antibody to PMM2免疫原：Recombinant full length protein of human PMM2纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：PMM2相关名称：Phosphomannomutase 2; PMM 2
基因编号(人)：
5373;
基因编号(小鼠)：
54128;
蛋白编号(人)：
O15305;
蛋白编号(小鼠)：
Q9Z2M7;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of PMM2 expression in A549 (A), Hela (B), NIH3T3 (C), mouse liver (D), rat brain (E) whole cell lysates. (Predicted band size: 13; 28 kD; Observed band size: 28 kD) -
Immunofluorescent analysis of PMM2 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).