产品描述：Rabbit polyclonal antibody to Histone H3 (MonoMethyl-R26)免疫原：KLH-conjugated synthetic Mono-methylated peptide corresponding to residues surrounding R26 of human Histone H3 protein. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IP (1/10 - 1/50)基因名称：HIST3H3相关名称：H3FT; Histone H3.1t; H3/t; H3t; H3/g 基因编号(人)： 8290; 蛋白编号(人)： Q16695; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histone H3 (MonoMethyl-R26) expression in Hela (A) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)
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  Immunofluorescent analysis of Histone H3 (MonoMethyl-R26) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.