产品描述：Rabbit polyclonal antibody to NFAT1免疫原：KLH-conjugated synthetic peptide of human NFAT1纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称：NFATC2相关名称：NFAT1; NFATP; Nuclear factor of activated T-cells, cytoplasmic 2; NF-ATc2; NFATc2; NFAT pre-existing subunit; NF-ATp; T-cell transcription factor NFAT1 基因编号(人)： 4773; 基因编号(小鼠)： 18019; 蛋白编号(人)： Q13469; 蛋白编号(小鼠)： Q60591; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NFAT1 expression in mouse heart (A) whole cell lysates. (Predicted band size: 76; 97; 98; 99; 100 kD; Observed band size: 140 kD)
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  Immunofluorescent analysis of NFAT1 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).