产品描述:Rabbit polyclonal antibody to OCTN2免疫原:Recombinant full length protein of human OCTN2纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)基因名称:SLC22A5相关名称:OCTN2; Solute carrier family 22 member 5; High-affinity sodium-dependent carnitine cotransporter; Organic cation/carnitine transporter 2
基因编号(人):
    6584;
基因编号(小鼠):
    20520;
基因编号(大鼠):
    29726;
蛋白编号(人):
    O76082;
蛋白编号(小鼠):
    Q9Z0E8;
蛋白编号(大鼠):
    O70594;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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             Western blot analysis of OCTN2 expression in MCF7 (A), A549 (B), mouse kidney (C) whole cell lysates. (Predicted band size: 24; 62; 65 kD; Observed band size: 80 kD) Western blot analysis of OCTN2 expression in MCF7 (A), A549 (B), mouse kidney (C) whole cell lysates. (Predicted band size: 24; 62; 65 kD; Observed band size: 80 kD)
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             Immunofluorescent analysis of OCTN2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. Immunofluorescent analysis of OCTN2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.


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