产品描述:Rabbit polyclonal antibody to CCR10免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CCR10. The exact sequence is proprietary.纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称:ACKR2相关名称:CCBP2; CCR10; CMKBR9; D6; Atypical chemokine receptor 2; C-C chemokine receptor D6; Chemokine receptor CCR-10; Chemokine receptor CCR-9; Chemokine-binding protein 2; Chemokine-binding protein D6
基因编号(人):
1238;
基因编号(小鼠):
59289;
蛋白编号(人):
O00590;
蛋白编号(小鼠):
O08707;
蛋白编号(大鼠):
O09027;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CCR10 expression in mouse liver (A), mouse kidney (B), rat liver (C) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 43 kD)
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Immunofluorescent analysis of CCR10 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.