产品描述：Rabbit polyclonal antibody to BWR1A免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human BWR1A. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)基因名称：SLC22A18相关名称：BWR1A; BWSCR1A; HET; IMPT1; ITM; ORCTL2; SLC22A1L; TSSC5; Solute carrier family 22 member 18; Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene A protein; Efflux transporter-like protein; Imprinted multi-membrane-spanning polyspecific transporter-related protein 1; Organic cation transporter-like protein 2; ORCTL-2; Solute carrier family 22 member 1-like; Tumor-suppressing STF cDNA 5 protein; Tumor-suppressing subchromosomal transferable fragment candidate gene 5 protein; p45-Beckwith-Wiedemann region 1 A; p45-BWR1A 基因编号(人)： 5002; 基因编号(小鼠)： 18400; 基因编号(大鼠)： 309131; 蛋白编号(人)： Q96BI1; 蛋白编号(小鼠)： Q78KK3; 蛋白编号(大鼠)： Q6AY78; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of BWR1A expression in C6 (A), CT26 (B), Hela (C), A2780 (D) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 40; 44 kD)
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  Immunofluorescent analysis of BWR1A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.