产品描述:Rabbit polyclonal antibody to ATRX免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ATRX. The exact sequence is proprietary.纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)基因名称:ATRX相关名称:RAD54L; XH2; Transcriptional regulator ATRX; ATP-dependent helicase ATRX; X-linked helicase II; X-linked nuclear protein; XNP; Znf-HX
基因编号(人):
546;
基因编号(小鼠):
22589;
蛋白编号(人):
P46100;
蛋白编号(小鼠):
Q61687;
蛋白编号(大鼠):
P70486;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ATRX expression in 3T3L1 (A), C6 (B), SGC7901 (C), MCF7 (D), EC9706 (E) whole cell lysates. (Predicted band size: 282 kD; Observed band size: 285 kD)
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Immunofluorescent analysis of ATRX staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.