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CPA5833
  • Western blot analysis of PERK (Phospho-T982) expression in A375 (A), MCF7 (B), mouse testis (C) whole cell lysates. (Predicted band size: 125 kD; Observed band size: 140 kD)
  • Immunohistochemical analysis of PERK (Phospho-T982) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
产品名称:Anti-PERK (Phospho-T982) Antibody
货号:CPA5833
来源:Rabbit
反应物种:H, M
实验应用:WB, IH
*反应物种注解:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*实验应用注解:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
规格
价格(元)
200 μl
4200
100 μl
2600
30 μl
1300
50 μl
1700
Ship in 3 days
产品描述:Rabbit polyclonal antibody to PERK (Phospho-T982)
免疫原:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T982 of human PERK protein. The exact sequence is proprietary.
纯化方式:The antibody was purified by immunogen affinity chromatography.
克隆类型:Polyclonal
产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
稀释比:WB (1/500 - 1/1000), IH (1/100 - 1/200)
基因名称:EIF2AK3
相关名称:PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK
基因编号(人): 9451;
基因编号(小鼠): 13666;
基因编号(大鼠): 29702;
蛋白编号(人): Q9NZJ5;
蛋白编号(小鼠): Q9Z2B5;
蛋白编号(大鼠): Q9Z1Z1;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of PERK (Phospho-T982) expression in A375 (A), MCF7 (B), mouse testis (C) whole cell lysates. (Predicted band size: 125 kD; Observed band size: 140 kD)
  • Immunohistochemical analysis of PERK (Phospho-T982) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Phillygenin ameliorates tight junction proteins reduction, fibrosis, and apoptosis in mice with chronic colitis via TGR5-mediated PERK-eIF2α-Ca2+ pathway
Journal Journal of Pharmaceutical Analysis
IF 6.1
Application WB
Reactivity Human
PMID
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