产品描述：Rabbit polyclonal antibody to Caspase 9 (Phospho-Y153)免疫原：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y153 of human Caspase 9 protein. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)基因名称：CASP9相关名称：MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6 基因编号(人)： 842; 蛋白编号(人)： P55211; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Caspase 9 (Phospho-Y153) expression in Hela (A), A549 (B) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 47 kD)
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  Immunofluorescent analysis of Caspase 9 (Phospho-Y153) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.