产品描述：Rabbit polyclonal antibody to ITPKA免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ITPKA. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)基因名称：ITPKA相关名称：Inositol-trisphosphate 3-kinase A; Inositol 145-trisphosphate 3-kinase A; IP3 3-kinase A; IP3K A; InsP 3-kinase A 基因编号(人)： 3706; 基因编号(小鼠)： 228550; 基因编号(大鼠)： 81677; 蛋白编号(人)： P23677; 蛋白编号(小鼠)： Q8R071; 蛋白编号(大鼠)： P17105; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ITPKA expression in A549 (A), LO2 (B) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 51 kD)
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  Immunofluorescent analysis of ITPKA staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.