产品描述：Rabbit polyclonal antibody to GPR37L1免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GPR37L1. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：GPR37L1相关名称：ETBRLP2; Prosaposin receptor GPR37L1; Endothelin B receptor-like protein 2; ETBR-LP-2; G-protein coupled receptor 37-like 1 基因编号(人)： 9283; 蛋白编号(人)： O60883; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GPR37L1 expression in SKOVCAR3 (A), HEK293T (B), A549 (C), LO2 (D), EC9706 (E) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 60 kD)
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  Immunofluorescent analysis of GPR37L1 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.