产品描述：Rabbit polyclonal antibody to Alpha-1A Adrenergic Receptor免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Alpha-1A Adrenergic Receptor. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)基因名称：ADRA1A相关名称：ADRA1C; Alpha-1A adrenergic receptor; Alpha-1A adrenoreceptor; Alpha-1A adrenoceptor; Alpha-1C adrenergic receptor; Alpha-adrenergic receptor 1c 基因编号(人)： 148; 基因编号(小鼠)： 11549; 基因编号(大鼠)： 29412; 蛋白编号(人)： P35348; 蛋白编号(小鼠)： P97718; 蛋白编号(大鼠)： P43140; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Alpha-1A Adrenergic Receptor expression in HepG2 (A), K562 (B) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 51 kD)
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  Immunofluorescent analysis of Alpha-1A Adrenergic Receptor staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).