产品描述：Rabbit polyclonal antibody to MYPT1 (Phospho-T853)免疫原：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T853 of human MYPT1 protein. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IH (1/100 - 1/200)基因名称：PPP1R12A相关名称：MBS; MYPT1; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit 基因编号(人)： 4659; 基因编号(小鼠)： 17931; 基因编号(大鼠)： 116670; 蛋白编号(人)： O14974; 蛋白编号(小鼠)： Q9DBR7; 蛋白编号(大鼠)： Q10728; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MYPT1 (Phospho-T853) expression in C6 (A), MCF7 (B) whole cell lysates. (Predicted band size: 115 kD; Observed band size: 130 kD)
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  Immunohistochemical analysis of MYPT1 (Phospho-T853) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.