产品描述:Rabbit polyclonal antibody to CD210a免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD210a. The exact sequence is proprietary.纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)基因名称:IL10RA相关名称:IL10R; Interleukin-10 receptor subunit alpha; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; CDw210a; Interleukin-10 receptor subunit 1; IL-10R subunit 1; IL-10R1; CD210
基因编号(人):
3587;
基因编号(小鼠):
16154;
蛋白编号(人):
Q13651;
蛋白编号(小鼠):
Q61727;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD210a expression in mouse heart (A), rat heart (B) whole cell lysates. (Predicted band size: 63 kD; Observed band size: 100 kD)
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Immunofluorescent analysis of CD210a staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).