产品描述：Rabbit polyclonal antibody to CD363 (Phospho-T236)免疫原：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T236 of human CD363 protein. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：S1PR1相关名称：CHEDG1; EDG1; Sphingosine 1-phosphate receptor 1; S1P receptor 1; S1P1; Endothelial differentiation G-protein coupled receptor 1; Sphingosine 1-phosphate receptor Edg-1; S1P receptor Edg-1; CD363 基因编号(人)： 1901; 基因编号(小鼠)： 13609; 基因编号(大鼠)： 29733; 蛋白编号(人)： P21453; 蛋白编号(小鼠)： O08530; 蛋白编号(大鼠)： P48303; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD363 (Phospho-T236) expression in HepG2 UV-treated (A), mouse brain (B) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 42 kD)
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  Immunofluorescent analysis of CD363 (Phospho-T236) staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.