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CPA4473
  • Western blot analysis of mTOR expression in HEK293T (A), NIH3T3 (B) whole cell lysates. (Predicted band size: 288 kD; Observed band size: 289 kD)
  • Immunohistochemical analysis of mTOR staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
产品名称:Anti-mTOR Antibody
货号:CPA4473
来源:Rabbit
反应物种:H, M, R, S
实验应用:WB, IH
*反应物种注解:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*实验应用注解:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
规格
价格(元)
200 μl
3500
100 μl
2200
30 μl
1100
50 μl
1500
Ship in 3 days
产品描述:Rabbit polyclonal antibody to mTOR
免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human mTOR. The exact sequence is proprietary.
纯化方式:The antibody was purified by immunogen affinity chromatography.
克隆类型:Polyclonal
产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
稀释比:WB (1/500 - 1/1000), IH (1/100 - 1/200)
基因名称:MTOR
相关名称:FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1
基因编号(人): 2475;
基因编号(小鼠): 56717;
基因编号(大鼠): 56718;
蛋白编号(人): P42345;
蛋白编号(小鼠): Q9JLN9;
蛋白编号(大鼠): P42346;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of mTOR expression in HEK293T (A), NIH3T3 (B) whole cell lysates. (Predicted band size: 288 kD; Observed band size: 289 kD)
  • Immunohistochemical analysis of mTOR staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Zi-Su-Zi decoction improves airway hyperresponsiveness in cough-variant asthma rat model through PI3K/AKT1/mTOR, JAK2/STAT3 and HIF-1α/NF-κB signaling pathways
Journal J Ethnopharmacol
IF 5.4
Application WB
Reactivity Rat
PMID 37187363
RLIG1 promotes triple-negative breast cancer proliferation by activating mTORC1 signal pathway by interacting with ERK
Journal Cellular Signalling
IF 3.7
Application IH
Reactivity Human
PMID
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