产品描述：Rabbit polyclonal antibody to CD61 (Phospho-Y785)免疫原：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y785 of human CD61 protein. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：ITGB3相关名称：GP3A; Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD61 基因编号(人)： 3690; 基因编号(小鼠)： 16416; 蛋白编号(人)： P05106; 蛋白编号(小鼠)： O54890; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD61 (Phospho-Y785) expression in U87MG (A), PC3 (B) whole cell lysates. (Predicted band size: 87 kD; Observed band size: 110 kD)
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  Immunofluorescent analysis of CD61 (Phospho-Y785) staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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  Direct ELISA antibody dose-response curve using Anti-CD61 (Phospho-Y785) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.