产品描述:Rabbit polyclonal antibody to WNK1 (Phospho-T60)免疫原:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T60 of human WNK1 protein. The exact sequence is proprietary.纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/1000), IH (1/100 - 1/200)基因名称:WNK1相关名称:HSN2; KDP; KIAA0344; PRKWNK1; Serine/threonine-protein kinase WNK1; Erythrocyte 65 kDa protein; p65; Kinase deficient protein; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; hWNK1
基因编号(人):
65125;
基因编号(小鼠):
232341;
基因编号(大鼠):
116477;
蛋白编号(人):
Q9H4A3;
蛋白编号(小鼠):
P83741;
蛋白编号(大鼠):
Q9JIH7;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of WNK1 (Phospho-T60) expression in HEK293T EGF-treated (A), Jurkat EGF-treated (B) whole cell lysates. (Predicted band size: 250 kD; Observed band size: 230 kD)
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Immunohistochemical analysis of WNK1 (Phospho-T60) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Activation of PI3-Kinase Stimulates Endocytosis of ROMK via Akt1/SGK1-Dependent Phosphorylation of WNK1