产品描述：Rabbit polyclonal antibody to VDR (Phospho-S208)免疫原：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S208 of human VDR protein. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)基因名称：VDR相关名称：NR1I1; Vitamin D3 receptor; VDR; 1,25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1 基因编号(人)： 7421; 基因编号(小鼠)： 22337; 基因编号(大鼠)： 24873; 蛋白编号(人)： P11473; 蛋白编号(小鼠)： P48281; 蛋白编号(大鼠)： P13053; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of VDR (Phospho-S208) expression in HCT116 (A), HEK293T (B) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 43 kD)
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  Immunofluorescent analysis of VDR (Phospho-S208) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).