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CPA4171
  • Western blot analysis of PKC mu (Phospho-Y463) expression in HeLa (A) whole cell lysates. (Predicted band size: 101 kD; Observed band size: 115 kD)
  • Immunohistochemical analysis of PKC mu (Phospho-Y463) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
产品名称:Anti-PKC mu (Phospho-Y463) Antibody
货号:CPA4171
来源:Rabbit
反应物种:H, M, R, D, Mk
实验应用:WB, IH
*反应物种注解:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*实验应用注解:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
规格
价格(元)
200 μl
4200
100 μl
2600
30 μl
1300
50 μl
1700
Ship in 3 days
产品描述:Rabbit polyclonal antibody to PKC mu (Phospho-Y463)
免疫原:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y463 of human PKC mu protein. The exact sequence is proprietary.
纯化方式:The antibody was purified by immunogen affinity chromatography.
克隆类型:Polyclonal
产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
稀释比:WB (1/500 - 1/1000), IH (1/100 - 1/200)
基因名称:PRKD1
相关名称:PKD; PKD1; PRKCM; Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu
基因编号(人): 5587;
基因编号(小鼠): 18760;
基因编号(大鼠): 85421;
蛋白编号(人): Q15139;
蛋白编号(小鼠): Q62101;
蛋白编号(大鼠): Q9WTQ1;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of PKC mu (Phospho-Y463) expression in HeLa (A) whole cell lysates. (Predicted band size: 101 kD; Observed band size: 115 kD)
  • Immunohistochemical analysis of PKC mu (Phospho-Y463) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Ultraviolet B irradiation and activation of protein kinase D in primary mouse epidermal keratinocytes
Journal Oncogene
IF 8
Application WB
Reactivity Mouse
PMID 21132013
Tyrosine phosphorylation of protein kinase D2 mediates ligand-inducible elimination of the Type 1 interferon receptor
Journal J Biol Chem
IF 4.8
Application WB
Reactivity Human
PMID 21865166
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