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CPA4162
  • Western blot analysis of POLR2E expression in mouse kidney (A) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 24 kD)
  • Immunohistochemical analysis of POLR2E staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
产品名称:Anti-POLR2E Antibody
货号:CPA4162
来源:Rabbit
反应物种:H, M, R, B, Mk
实验应用:WB, IH
*反应物种注解:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*实验应用注解:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
规格
价格(元)
200 μl
3500
100 μl
2200
30 μl
1100
50 μl
1500
Ship in 3 days
产品描述:Rabbit polyclonal antibody to POLR2E
免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human POLR2E. The exact sequence is proprietary.
纯化方式:The antibody was purified by immunogen affinity chromatography.
克隆类型:Polyclonal
产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
稀释比:WB (1/500 - 1/1000), IH (1/100 - 1/200)
基因名称:POLR2E
相关名称:DNA-directed RNA polymerases I, II, and III subunit RPABC1; RNA polymerases I, II, and III subunit ABC1; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; RPB5 homolog; XAP4
基因编号(人): 5434;
基因编号(小鼠): 66420;
基因编号(大鼠): 690966;
蛋白编号(人): P19388;
蛋白编号(小鼠): Q80UW8;
蛋白编号(大鼠): B0BNE2;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of POLR2E expression in mouse kidney (A) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 24 kD)
  • Immunohistochemical analysis of POLR2E staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Regulation of Androgen Receptor-Mediated Transcription by RPB5 Binding Protein URI/RMP
Journal Mol Cell Biol
IF 5.3
Application WB
Reactivity Human
PMID 21730289
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