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CPA4106
  • Western blot analysis of IDOL expression in Hela (A), COS7 (B), MCF7 (C), PC12 (D), CT26 (E) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 50 kD)
  • Immunohistochemical analysis of IDOL staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
产品名称:Anti-IDOL Antibody
货号:CPA4106
来源:Rabbit
反应物种:H, M, R, B, Mk
实验应用:WB, IH
*反应物种注解:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*实验应用注解:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
规格
价格(元)
200 μl
3500
100 μl
2200
30 μl
1100
50 μl
1500
Ship in 3 days
产品描述:Rabbit polyclonal antibody to IDOL
免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IDOL. The exact sequence is proprietary.
纯化方式:The antibody was purified by immunogen affinity chromatography.
克隆类型:Polyclonal
产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
稀释比:WB (1/500 - 1/1000), IH (1/100 - 1/200)
基因名称:MYLIP
相关名称:BZF1; IDOL; E3 ubiquitin-protein ligase MYLIP; Inducible degrader of the LDL-receptor; Idol; Myosin regulatory light chain interacting protein; MIR
基因编号(人): 29116;
基因编号(小鼠): 218203;
蛋白编号(人): Q8WY64;
蛋白编号(小鼠): Q8BM54;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of IDOL expression in Hela (A), COS7 (B), MCF7 (C), PC12 (D), CT26 (E) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 50 kD)
  • Immunohistochemical analysis of IDOL staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Reduced VLDL clearance in Apoe(-/-)Npc1(-/-) mice is associated with increased Pcsk9 and Idol expression and decreased hepatic LDL-receptor levels
Journal J Lipid Res
IF 6.5
Application WB
Reactivity Mouse
PMID 20562239
LXRß activation increases intestinal cholesterol absorption, leading to an atherogenic lipoprotein profile
Journal J Intern Med
IF 39
Application WB
Reactivity Mouse
PMID 22329358
Fibroblast Growth Factor-21 (FGF21) Regulates Low-density Lipoprotein Receptor (LDLR) Levels in Cells via the E3-ubiquitin Ligase Mylip/Idol and the Canopy2 (Cnpy2)/Mylip-interacting Saposin-like Protein (Msap)
Journal J Biol Chem
IF 4.8
Application WB
Reactivity Mouse
PMID 22378787
IDOL stimulates clathrin-independent endocytosis and multivesicular body-mediated lysosomal degradation of the low-density lipoprotein receptor
Journal Mol Cell Biol
IF 5.3
Application WB
Reactivity Human
PMID 23382078
Reciprocal regulation of very low density lipoprotein receptors (VLDLRs) in neurons by brain-derived neurotrophic factor (BDNF) and Reelin: involvement of the E3 ligase Mylip/Idol
Journal J Biol Chem
IF 4.8
Application WB
Reactivity Rat
PMID 23990472
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