产品描述：Rabbit polyclonal antibody to Histone H3 (Acetyl-K23)免疫原：KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K23 of human Histone H3 protein. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)基因名称：HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D; H3F3A; H3F3B相关名称：HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3 基因编号(人)： 8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968; 126961; 333932; 653604; 3020; 3021; 基因编号(小鼠)： 319152; 15077; 15078; 基因编号(大鼠)： 291159; 100361558; 蛋白编号(人)： P68431; Q71DI3; P84243; 蛋白编号(小鼠)： P68433; P84228; P84244; 蛋白编号(大鼠)： Q6LED0; P84245; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histone H3 (Acetyl-K23) expression in HEK293T (A), Hela (B) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)
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  Immunofluorescent analysis of Histone H3 (Acetyl-K23) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).