产品描述:Rabbit polyclonal antibody to GLURD2免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GLURD2. The exact sequence is proprietary.纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)基因名称:GRID2相关名称:GLURD2; Glutamate receptor ionotropic, delta-2; GluD2; GluR delta-2 subunit
基因编号(人):
2895;
基因编号(小鼠):
14804;
基因编号(大鼠):
79220;
蛋白编号(人):
O43424;
蛋白编号(小鼠):
Q61625;
蛋白编号(大鼠):
Q63226;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of GLURD2 expression in zebrafish (A) whole cell lysates. (Predicted band size: 113 kD; Observed band size: 113 kD)
-
Immunofluorescent analysis of GLURD2 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Neto2 interacts with the scaffolding protein GRIP and regulates synaptic abundance of kainate receptors