产品描述：Rabbit polyclonal antibody to GATA3 (Phospho-S308)免疫原：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S308 of human GATA3 protein. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)基因名称：GATA3相关名称：Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3 基因编号(人)： 2625; 基因编号(小鼠)： 14462; 蛋白编号(人)： P23771; 蛋白编号(小鼠)： P23772; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GATA3 (Phospho-S308) expression in zebrafish (A) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 55; 40 kD)
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  Immunofluorescent analysis of GATA3 (Phospho-S308) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).