产品描述:Rabbit polyclonal antibody to TNFAIP8L1免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TNFAIP8L1. The exact sequence is proprietary.纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称:TNFAIP8L1相关名称:Tumor necrosis factor alpha-induced protein 8-like protein 1; TNF alpha-induced protein 8-like protein 1; TNFAIP8-like protein 1
基因编号(人):
126282;
基因编号(小鼠):
66443;
蛋白编号(人):
Q8WVP5;
蛋白编号(小鼠):
Q8K288;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TNFAIP8L1 expression in AML12 (A), A549 (B), HepG2 (C) whole cell lysates. (Predicted band size: 20 kD; Observed band size: 21 kD)
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Immunofluorescent analysis of TNFAIP8L1 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).