产品描述：Rabbit polyclonal antibody to HLA-DQB1免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HLA-DQB1. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200)基因名称：HLA-DQB1; HLA-DQB2相关名称：HLA-DQB1; HLA-DQB; HLA class II histocompatibility antigen, DQ beta 1 chain; MHC class II antigen DQB1; HLA-DQB2; HLA-DXB; HLA class II histocompatibility antigen, DQ beta 2 chain; HLA class II histocompatibility antigen, DX beta chain; MHC class II antigen DQB2 基因编号(人)： 100507714; 蛋白编号(人)： P01920; P05538; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HLA-DQB1 expression in mouse lung (A), mouse spleen (B), rat lung (C), rat spleen (D) whole cell lysates. (Predicted band size: 29; 30 kD; Observed band size: 30 kD)
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  Immunohistochemical analysis of HLA-DQB1 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of HLA-DQB1 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).