说明书
MSDS产品描述：Rabbit polyclonal antibody to GALR1免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GALR1. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：GALR1相关名称：GALNR; GALNR1; Galanin receptor type 1; GAL1-R; GALR-1
基因编号(人)：
2587;
基因编号(小鼠)：
14427;
基因编号(大鼠)：
50577;
蛋白编号(人)：
P47211;
蛋白编号(小鼠)：
P56479;
蛋白编号(大鼠)：
Q62805;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of GALR1 expression in LO2 (A), A2780 (B), THP1 (C), mouse brain (D), mouse liver (E), rat liver (F) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 43 kD) -
Immunofluorescent analysis of GALR1 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.