产品描述：Rabbit polyclonal antibody to HECW2免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HECW2. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)基因名称：HECW2相关名称：KIAA1301; NEDL2; E3 ubiquitin-protein ligase HECW2; HECT, C2 and WW domain-containing protein 2; NEDD4-like E3 ubiquitin-protein ligase 2 基因编号(人)： 57520; 基因编号(小鼠)： 329152; 蛋白编号(人)： Q9P2P5; 蛋白编号(小鼠)： Q6I6G8; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HECW2 expression in H1792 (A), A549 (B), PMVEC (C), AML12 (D) whole cell lysates. (Predicted band size: 175 kD; Observed band size: 175 kD)
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  Immunofluorescent analysis of HECW2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).