产品描述:Rabbit polyclonal antibody to p53 (Phospho-S37)免疫原:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S37 of human p53 protein. The exact sequence is proprietary.纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/1000), IH (1/100 - 1/200)基因名称:TP53相关名称:P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
基因编号(人):
7157;
蛋白编号(人):
P04637;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of p53 (Phospho-S37) expression in COLO205 (A), Jurkat UV-treated (B) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 53 kD)
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Immunohistochemical analysis of p53 (Phospho-S37) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.