产品描述：Rabbit polyclonal antibody to Delta-NaCH免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Delta-NaCH. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：SCNN1D相关名称：DNACH; Amiloride-sensitive sodium channel subunit delta; Delta-NaCH; Epithelial Na(+) channel subunit delta; Delta-ENaC; ENaCD; Nonvoltage-gated sodium channel 1 subunit delta; SCNED 基因编号(人)： 6339; 蛋白编号(人)： P51172; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Delta-NaCH expression in mouse lung (A), mouse muscle (B), rat testis (C), rat muscle (D) whole cell lysates. (Predicted band size: 87 kD; Observed band size: 70 kD)
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  Immunofluorescent analysis of Delta-NaCH staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.