产品描述：Rabbit polyclonal antibody to RPS6 (Phospho-S235)免疫原：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S235 of human RPS6 protein. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)基因名称：RPS6相关名称：40S ribosomal protein S6; Phosphoprotein NP33 基因编号(人)： 6194; 基因编号(小鼠)： 20104; 基因编号(大鼠)： 100911372; 29304; 蛋白编号(人)： P62753; 蛋白编号(小鼠)： P62754; 蛋白编号(大鼠)： P62755; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RPS6 (Phospho-S235) expression in HeLa Calyculin A-treated (A), NIH3T3 (B) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 32 kD)
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  Immunofluorescent analysis of RPS6 (Phospho-S235) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).