产品描述:Rabbit polyclonal antibody to Kv9.2免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Kv9.2. The exact sequence is proprietary.纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称:KCNS2相关名称:KIAA1144; Potassium voltage-gated channel subfamily S member 2; Delayed-rectifier K(+) channel alpha subunit 2; Voltage-gated potassium channel subunit Kv9.2
基因编号(人):
3788;
基因编号(小鼠):
16539;
基因编号(大鼠):
66022;
蛋白编号(人):
Q9ULS6;
蛋白编号(小鼠):
O35174;
蛋白编号(大鼠):
Q9ER26;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Kv9.2 expression in A2780 (A) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 54 kD)
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Immunofluorescent analysis of Kv9.2 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).