产品描述：Rabbit polyclonal antibody to HER2免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human HER2. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IH (1/50 - 1/100)基因名称：ERBB2相关名称：HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340 基因编号(人)： 2064; 基因编号(小鼠)： 13866; 蛋白编号(人)： P04626; 蛋白编号(小鼠)： P70424; 蛋白编号(大鼠)： P06494; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HER2 expression in MCF7 (A), HCT116 (B), SGC7901 (C) whole cell lysates. (Predicted band size: 137 kD; Observed band size: 185; 137 kD)
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  Immunohistochemical analysis of HER2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.