产品描述:Rabbit polyclonal antibody to Cytochrome P450 2A6免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Cytochrome P450 2A6. The exact sequence is proprietary.纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)基因名称:CYP2A6相关名称:CYP2A3; Cytochrome P450 2A6; 1,4-cineole 2-exo-monooxygenase; CYPIIA6; Coumarin 7-hydroxylase; Cytochrome P450 IIA3; Cytochrome P450(I)
基因编号(人):
1548;
蛋白编号(人):
P11509;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Cytochrome P450 2A6 expression in rat liver (A) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 50 kD)
-
Immunofluorescent analysis of Cytochrome P450 2A6 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).