产品描述：Rabbit polyclonal antibody to RAPH1免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RAPH1. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：RAPH1相关名称：ALS2CR18; ALS2CR9; KIAA1681; LPD; PREL2; RMO1; Ras-associated and pleckstrin homology domains-containing protein 1; RAPH1; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein; Lamellipodin; Proline-rich EVH1 ligand 2; PREL-2; Protein RMO1 基因编号(人)： 65059; 蛋白编号(人)： Q70E73; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RAPH1 expression in HepG2 (A), H9C2 (B) whole cell lysates. (Predicted band size: 135 kD; Observed band size: 135 kD)
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  Immunofluorescent analysis of RAPH1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).