说明书
MSDS产品描述：Rabbit polyclonal antibody to p47 phox (Phospho-S370)免疫原：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S370 of human p47 phox protein. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IH (1/50 - 1/100)基因名称：NCF1相关名称：NOXO2; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox
基因编号(人)：
653361;
基因编号(小鼠)：
17969;
蛋白编号(人)：
P14598;
蛋白编号(小鼠)：
Q09014;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of p47 phox (Phospho-S370) expression in HEK293T (A), mouse lung (B), mouse liver (C), rat lung (D), rat liver (E) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 47 kD) -
Immunohistochemical analysis of p47 phox (Phospho-S370) staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Direct ELISA antibody dose-response curve using Anti-p47 phox (Phospho-S370) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.