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CPA2888
  • Western blot analysis of CADM4 expression in NCIH226 (A) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 43 kD)
  • Immunohistochemical analysis of CADM4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of CADM4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
产品名称:Anti-CADM4 Antibody
货号:CPA2888
来源:Rabbit
反应物种:H, M, R
实验应用:WB, IH, IF/IC, IP
*反应物种注解:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*实验应用注解:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
规格
价格(元)
200 μl
3500
100 μl
2200
30 μl
1100
50 μl
1500
Ship in 3 days
产品描述:Rabbit polyclonal antibody to CADM4
免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CADM4. The exact sequence is proprietary.
纯化方式:The antibody was purified by immunogen affinity chromatography.
克隆类型:Polyclonal
产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
稀释比:WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200), IP (1/10 - 1/100)
基因名称:CADM4
相关名称:IGSF4C; NECL4; TSLL2; Cell adhesion molecule 4; Immunoglobulin superfamily member 4C; IgSF4C; Nectin-like protein 4; NECL-4; TSLC1-like protein 2
基因编号(人): 199731;
基因编号(小鼠): 260299;
基因编号(大鼠): 365216;
蛋白编号(人): Q8NFZ8;
蛋白编号(小鼠): Q8R464;
蛋白编号(大鼠): Q1WIM1;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of CADM4 expression in NCIH226 (A) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 43 kD)
  • Immunohistochemical analysis of CADM4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of CADM4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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