说明书
MSDS产品描述：Rabbit polyclonal antibody to LRP11免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human LRP11. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IP (1/10 - 1/100)基因名称：LRP11相关名称：Low-density lipoprotein receptor-related protein 11; LRP-11
基因编号(人)：
84918;
蛋白编号(人)：
Q86VZ4;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of LRP11 expression in A431 (A), HepG2 (B), Jurkat (C) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 49 kD) -
Immunoprecipitation of LRP11 from 0.5mg Jurkat whole cell extract lysate, using 5ug of Anti-LRP11 Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with Anti-LRP11 Antibody.